Sodium from green beans

Identity: Ashni Couprie and Dennise Susanto

Course: 5c


From previous experiments we now have learned that salt is taken out from green beans. Being more specific: sodium is removed from if the beans happen to be cooked in water through which salt has become dissolved. This kind of experiment is based upon the osmoses theory. According to this theory the cell walls of the green beans happen to be what we call a semi permeable membrane. This membrane simply lets water through and not the element that is mixed into the drinking water, as in the case is NaCl. Because the attention of the sodium inside the cell and in the water is different equalization will happen. You can expect which the cells will require more water inside within this experiment, so the concentration of salt in the water improvements because there is the same amount of salt in much less water. You could ask why the coffee beans are staying cooked in water which usually contains salt, when the espresso beans themselves currently contain salt. In this way we have came to the following inquiry problem on which this experiment have been based.

Inquiry Issue: What is the relation between amount of salt taken out from the green beans, when being cooked, and the volume of mol NaCl mixed in this drinking water?

Hypothesis: We expect them to be dependent from each other. If even more salt is definitely dissolved in the water the cell may have take in less water. In the event that less salt is blended in the drinking water the cellular material will have to take in more drinking water, so the focus will vary far more.

The attentiveness of sodium in the normal water will have to be measured in this test. We is going to do this simply by titration. Just for this we will use a pipette, a burette and a great Erlenmeyer. Prior to we can use these we must clean all of them first simply by rinsing these people three times with all the same liquid. The flacon will have to be browse very specifically so all of the drops in the top of the flacon will be lost.

Trial and error design

Musical instruments


* burette (50mL)

5. pipette (10mL)

* dropper tube (indicator)

* cooking stones

* burner

5. lighter

* tripod

* wire mesh

* large beaker (500mL)

* tiny beaker (50mL)

* Erlenmeyer (250mL)

* weighing machine (2 decimals)

* home foil

* cutting board

2. knife

2. thermometer

5. stopwatch

* stirring rod


* Unadulterated water

* NaCl-solution 0, 100 M NaCl

* AgNO3 0, 05 M/L

* KCrO4

* green beans

There is a standard answer of zero, 100M NaCl with which the experiment starts. Starting here this remedy will be thinned. We start with adding twenty ml unadulterated water and extracting 20ml from the standard solution. In this manner the total amount will remain the same (250 ml). The solutions change between 250ml 0, 100M NaCl and 0ml unadulterated water and 150ml zero, 100M NaCl en 100ml distilled drinking water. The beans will be lower into bits of 1cm. Weighing them provides an amount of 25. 09 grams. Each time a solution of precisely 250ml will be used. The solution will be heated in beaker that has been closed with kitchen foil. If a temperature of 80 degrees can be reached due to heating the, the espresso beans that has been considered will be devote that beaker and will be shut down again. This will likely be heated up 5 minutes. The heating happens by placing blue not-rustling flame underneath the beaker. After 5 minutes heating, the liquefied will be poured into a Erlenmeyer. In this way the different solutions will be made that furthermore will probably be examined. Then 10, 00ml of the option from the Erlenmeyer will be pipetted and what remains can be thrown away. Through this NaCl remedy with unidentified concentration 10 drops from the indicator KCrO4 will be added. The burette will be filled up with AgNO3. The beginning and end values will be read out so the sum AgNO3 applied is known. In the event the solution in the Erlenmeyer that was yellow-colored, has switched red, the titration is completed. Results

Stand 1: Measurements processed.

Starting value burette(ml)| End value burette(ml)| Amount AgNO3...



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